Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm 
resolution.

Göttfert, F.; Wurm, C. A.; Müller, V.; Berning, S.; Cordes, V. C.; Honigmann, A.; Hell, S. W.

doi:10.1016/j.bpj.2013.05.029

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Journal Article

Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution.

MPS-Authors

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Göttfert, F.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons16058

Wurm, C. A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons40296

Müller, V.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons45995

Berning, S.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons14955

Cordes, V. C.
Research Group of Nuclear Architecture, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons40292

Honigmann, A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15210

Hell, S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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http://www.sciencedirect.com/science?_ob=MiamiImageURL&_cid=277708&_user=38661&_pii=S0006349513006127&_check=y&_origin=article&_zone=toolbar&_coverDate=02-Jul-2013&view=c&originContentFamily=serial&wchp=dGLzVlt-zSkWb&md5=77d87c04f02ef68fc327c7747b92b642&pid=1-s2.0-S0006349513006127-main.pdf
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Citation

Göttfert, F., Wurm, C. A., Müller, V., Berning, S., Cordes, V. C., Honigmann, A., et al. (2013). Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution. Biophysical Journal, 105(1), L01-L03. doi:10.1016/j.bpj.2013.05.029.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0013-E53F-2

Abstract

We report on a fiber laser-based stimulated emission-depletion microscope providing down to ~20 nm resolution in raw data images as well as 15–19 nm diameter probing areas in fluorescence correlation spectroscopy. Stimulated emission depletion pulses of nanosecond duration and 775 nm wavelength are used to silence two fluorophores simultaneously, ensuring offset-free colocalization analysis. The versatility of this superresolution method is exemplified by revealing the octameric arrangement of Xenopus nuclear pore complexes and by quantifying the diffusion of labeled lipid molecules in artificial and living cell membranes.