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In caco-2 cells, most of the "apical" SGLT1 resides in intracellular, microtubuli-associated vesicles

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Kipp,  Helmut
Sonstige Wissenschaftliche Organisationseinheiten, Max Planck Institute of Molecular Physiology, Max Planck Society;

Khoursandi,  Saeed
Max Planck Institute of Molecular Physiology, Max Planck Society;

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Kinne,  Rolf K. H.
Sonstige Wissenschaftliche Organisationseinheiten, Max Planck Institute of Molecular Physiology, Max Planck Society;

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Citation

Kipp, H., Khoursandi, S., & Kinne, R. K. H. (2002). In caco-2 cells, most of the "apical" SGLT1 resides in intracellular, microtubuli-associated vesicles.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0014-0EBA-A
Abstract
We investigated the distribution of the endogenous sodium/D-glucose cotransporter (SGLT1) in polarized Caco-2 cells, a model for enterocytes. A cellular organelle fraction was separated by free flow electrophoresis. As detected by an ELISA assay the major amount of SGLT1 resides in early endosomal fractions and only a minor amount in apical plasma membranes. The distribution-ratio between endosomes and apical membrane was approximately 2:1. Further immunochemical investigation of SGLT1 distribution by epifluorescence and confocal microscopy revealed that SGLT1 is located in small vesicles associated with microtubuli. Furthermore, the half-life of SGLT1 in Caco-2 cells was determined to be 2.5 d by metabolic labeling followed by immunoprecipitation. Since SGLT1 has a relatively long residence time, it is unlikely that intracellular SGLT1 populations are part of the synthesis/degradation pathway. We, therefore, propose that intracellular compartments containing SGLT1 are part of an endo-/exocytosis process which regulates SGLT1 abundance at the apical cell surface in response to altered physiological demands for D-glucose reabsorption. (This abstract is as it is printed in the journal).