Crystallization and preliminary X-ray diffraction analysis of the Rab escort 
protein-1 in complex with Rab geranylgeranyltransferase

Rak, Alexey; Reents, Reinhard; Pylypenko, Olena; Niculae, Anca; Sidorovitch, Vadim; Thomä, Nicolas H.; Waldmann, Herbert; Schlichting, Ilme; Goody, Roger S.; Alexandrov, Kirill

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Crystallization and preliminary X-ray diffraction analysis of the Rab escort protein-1 in complex with Rab geranylgeranyltransferase

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/persons/resource/persons98720

Rak, Alexey
Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society;

Reents, Reinhard
Max Planck Institute of Molecular Physiology, Max Planck Society;

Pylypenko, Olena
Max Planck Institute of Molecular Physiology, Max Planck Society;

Niculae, Anca
Max Planck Institute of Molecular Physiology, Max Planck Society;

Sidorovitch, Vadim
Max Planck Institute of Molecular Physiology, Max Planck Society;

Thomä, Nicolas H.
Max Planck Institute of Molecular Physiology, Max Planck Society;

/persons/resource/persons98735

Waldmann, Herbert
Abt. IV: Chemische Biologie, Max Planck Institute of Molecular Physiology, Max Planck Society;

/persons/resource/persons98726

Schlichting, Ilme
Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society;

/persons/resource/persons98693

Goody, Roger S.
Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society;

/persons/resource/persons98674

Alexandrov, Kirill
Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society;

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引用

Rak, A., Reents, R., Pylypenko, O., Niculae, A., Sidorovitch, V., Thomä, N. H., Waldmann, H., Schlichting, I., Goody, R. S., & Alexandrov, K. (2001). Crystallization and preliminary X-ray diffraction analysis of the Rab escort protein-1 in complex with Rab geranylgeranyltransferase. Journal of Structural Biology, 136(2):, pp. 158-161. Retrieved from http://dx.doi.org/10.1006/jsbi.2001.4433.

引用: https://hdl.handle.net/11858/00-001M-0000-0014-0F85-9

要旨

Posttranslational prenylation of proteins is a widespread phenomenon and the majority of prenylated proteins are geranylgeranylated members of the Rab GTPase family. Geranylgeranylation is catalyzed by Rab geranylgeranyltransferase (RabGGTase) and is critical for the ability of Rab protein to mediate vesicular docking and fusion of various intracellular vesicles. RabGGTase consists of a catalytic alpha/beta heterodimer and an accessory protein termed Rab escort protein (REP-1) that delivers the newly prenylated Rab proteins to their target membrane. Mutations in the REP-I gene in humans lead to an X-chromosome-linked defect known as choroideremia-a debilitating disease that inevitably culminates in complete blindness. Here we report in vitro assembly and purification of the stoichiometric ternary complex of RabGGTase with REP-1 stabilized by a hydrolysis-resistant phosphoisoprenoid analog-farnesyl phosphonyl(methyl)phoshonate. The complex formed crystals of extended plate morphology under low ionic-strength conditions. X-ray diffraction data were collected to 2.8 Angstrom resolution at the ESRF. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 68.7, b = 197.7, c = 86.1 Angstrom, beta = 113.4degrees. Preliminary structural analysis revealed the presence of one molecule in the asymmetric unit. (C) 2001 Elsevier Science (USA).