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Conference Paper

Signal perception and intracellular signal transduction in plant pathogen defense

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Zimmermann,  S.
Plant Signalling, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Citation

Nurnberger, T., Wirtz, W., Nennstiel, D., Hahlbrock, K., Jabs, T., Zimmermann, S., et al. (1997). Signal perception and intracellular signal transduction in plant pathogen defense. In 7th Swiss Workshop of Methodology in Receptor Research (pp. 127-136).


Cite as: https://hdl.handle.net/11858/00-001M-0000-0014-3116-5
Abstract
Disease resistance in plant/pathogen interactions requires sensitive and specific recognition mechanisms for pathogen-derived signals in plants. Cultured parsley (Petroselinum crispum) cells respond to treatment with a crude cell wall preparation derived from the phytopathogenic fungus Phytophthora sojae with transcriptional activation of the same set of defense-related genes as are activated in parsley leaves upon infection with fungal spores. A 13 amino acid core sequence (Pep-13) of a 42 kDa fungal cell wall glycoprotein was identified which stimulates the same responses as the crude cell wall elicitor, namely macroscopic Ca2+ and H+-influxes, effluxes of K+- and Cl- ions, production of active oxygen species (oxidative burst), defense-related gene activation, and formation of antifungal phytoalexins. Using [I-125]Tyr-Pep-13 as ligand in binding assays, a single-class high-affinity binding site in parsley microsomal membranes and protoplasts could be detected. Binding was specific, saturable, and reversible. By chemical crosslinking, a 91 kDa parsley plasma membrane protein was identified to be the receptor of the peptide elicitor. Isolation of this receptor protein involved in pathogen defense in plants is under way.