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Journal Article

Combinatorial nucleoside-deletion-scanning mutagenesis of functional DNA.

MPS-Authors
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Samanta,  B.
Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society;

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Höbartner,  C.
Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society;

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1835589_Supplement_1.pdf
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Citation

Samanta, B., & Höbartner, C. (2013). Combinatorial nucleoside-deletion-scanning mutagenesis of functional DNA. Angewandte Chmie International Edition, 52(10), 2995-2999. doi:10.1002/anie.201208103.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0014-4E93-6
Abstract
Compact and informative: Individual nucleosides are statistically mutated by replacement with a non-nucleosidic spacer unit Δ that encodes the “deletion” in a single DNA library. This efficient mutagenesis approach enables minimization of functional DNA, identifying essential nucleotides, as demonstrated for two nucleic-acid ligating deoxyribozymes.