Combinatorial nucleoside-deletion-scanning mutagenesis of functional DNA.

Samanta, B.; Höbartner, C.

doi:10.1002/anie.201208103

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Combinatorial nucleoside-deletion-scanning mutagenesis of functional DNA.

MPS-Authors

/persons/resource/persons101693

Samanta, B.
Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15234

Höbartner, C.
Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society;

External Resource

http://onlinelibrary.wiley.com/doi/10.1002/anie.201208103/pdf
(Publisher version)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

1835589_Supplement_1.pdf
(Supplementary material), 5MB

Citation

Samanta, B., & Höbartner, C. (2013). Combinatorial nucleoside-deletion-scanning mutagenesis of functional DNA. Angewandte Chmie International Edition, 52(10), 2995-2999. doi:10.1002/anie.201208103.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0014-4E93-6

Abstract

Compact and informative: Individual nucleosides are statistically mutated by replacement with a non-nucleosidic spacer unit Δ that encodes the “deletion” in a single DNA library. This efficient mutagenesis approach enables minimization of functional DNA, identifying essential nucleotides, as demonstrated for two nucleic-acid ligating deoxyribozymes.