Efficient band-selective homonuclear CO-CA cross-polarization in protonated 
proteins.

Chevelkov, V.; Shi, C.; Fasshuber, H. K.; Becker, S.; Lange, A.

doi:10.1007/s10858-013-9767-1

Datensatz

DATENSATZ AKTIONENEXPORT

Zur Ablage hinzufügen

Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben

Zeitschriftenartikel

Efficient band-selective homonuclear CO-CA cross-polarization in protonated proteins.

MPG-Autoren

/persons/resource/persons82487

Chevelkov, V.
Research Group of Solid-State NMR, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons82489

Fasshuber, H. K.
Research Group of Solid-State NMR, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons14824

Becker, S.
Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15414

Lange, A.
Research Group of Solid-State NMR, MPI for Biophysical Chemistry, Max Planck Society;

Externe Ressourcen

http://link.springer.com/content/pdf/10.1007%2Fs10858-013-9767-1.pdf
(Verlagsversion)

Volltexte (beschränkter Zugriff)

Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.

Volltexte (frei zugänglich)

1836824.pdf
(Verlagsversion), 584KB

Ergänzendes Material (frei zugänglich)

1836824_Supplement_1.docx
(Ergänzendes Material), 418KB

Zitation

Chevelkov, V., Shi, C., Fasshuber, H. K., Becker, S., & Lange, A. (2013). Efficient band-selective homonuclear CO-CA cross-polarization in protonated proteins. Journal of Biomolecular NMR, 56(4), 303-311. doi:10.1007/s10858-013-9767-1.

Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0014-55AB-4

Zusammenfassung

Previously introduced for highly deuterated proteins, band-selective magnetization transfer between CO and CA spins by dipolar-based homonuclear cross polarization is applied here to a protonated protein. Robust and efficient recoupling is achieved when the sum of effective radio-frequency fields on CO and CA resonances equals two times the spinning rate, yielding up to 33 % of magnetization transfer efficiency in protonated ubiquitin. The approach is designed for moderate magic-angle spinning rates and high external magnetic fields when the isotropic chemical shift difference of CO and CA considerably exceeds the spinning rate. This method has been implemented in N(i)CO(i-1)CA(i-1) and CA(i)(N-i)CO(i-1)CA(i-1) two-dimensional interresidual correlation experiments for fast and efficient resonance assignment of ubiquitin by solid-state NMR spectroscopy.