Spheroid formation of human thyroid cancer cells in an automated culturing 
system during the Shenzhou-8 Space mission

Pietsch, Jessica; Ma, Xiao; Wehland, Markus; Aleshcheva, Ganna; Schwarzwaelder, Achim; Segerer, Jurgen; Birlem, Maria; Horn, Astrid; Bauer, Johann; Infanger, Manfred; Grimm, Daniela

doi:10.1016/j.biomaterials.2013.06.054

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Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission

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Bauer, Johann
Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society;

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Pietsch, J., Ma, X., Wehland, M., Aleshcheva, G., Schwarzwaelder, A., Segerer, J., et al. (2013). Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission. BIOMATERIALS, 34(31), 7694-7705. doi:10.1016/j.biomaterials.2013.06.054.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0014-5BE3-7

Abstract

Human follicular thyroid cancer cells were cultured in Space to investigate the impact of microgravity on 3D growth. For this purpose, we designed and constructed a cell container that can endure enhanced physical forces, is connected to fluid storage chambers, performs media changes and cell harvesting automatically and supports cell viability. The container consists of a cell suspension chamber, two reserve tanks for medium and fixative and a pump for fluid exchange. The selected materials proved durable, non-cytotoxic, and did not inactivate RNAlater. This container was operated automatically during the unmanned Shenzhou-8 Space mission. FTC-133 human follicular thyroid cancer cells were cultured in Space for 10 days. Culture medium was exchanged after 5 days in Space and the cells were fixed after 10 days. The experiment revealed a scaffold-free formation of extraordinary large three-dimensional aggregates by thyroid cancer cells with altered expression of EGF and CTGF genes under real microgravity. (C) 2013 Elsevier Ltd. All rights reserved.