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Conference Paper

Nanoscopy with focused light.


Hell,  S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Hell, S. W. (2013). Nanoscopy with focused light. doi:10.1117/12.2032261.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0014-6A17-3
For many decades, it has been accepted that the resolution of a lens-based optical microscope is limited to about d =λ (2 NA) < 200 nm. The discovery in the 1990’s that elementary transitions between the states of a fluorophore can be used to eliminate the limiting role of diffraction has led to lens-based light microscopy concepts with resolution down to the nanometer scale1,2. Currently, all far-field fluorescence nanoscopy (superresolution) concepts that have found wider application share a common enabling element: they modulate the fluorescence capability of adjacent features such that they fluoresce sequentially3,4.