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Journal Article

A large pseudoautosomal region on the sex chromosomes of the frog Silurana tropicalis


Chain,  Frédéric J. J.
Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Bewick, A. J., Chain, F. J. J., Zimmerman, L. B., Sesay, A., Gilchrist, M. J., Owens, N. D., et al. (2013). A large pseudoautosomal region on the sex chromosomes of the frog Silurana tropicalis. Genome biology and evolution, 5(6), 1087-1098. doi:10.1093/gbe/evt073.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0014-6CDD-8
Sex chromosome divergence has been documented across phylogenetically diverse species, with amphibians typically having cytologically nondiverged (“homomorphic”) sex chromosomes. With an aim of further characterizing sex chromosome divergence of an amphibian, we used “RAD-tags” and Sanger sequencing to examine sex specificity and heterozygosity in theWestern clawed frog Silurana tropicalis (also known as Xenopus tropicalis). Our findings based on approximately 20 million genotype calls and approximately 200 polymerase chain reaction-amplified regions across multiple male and female genomes failed to identify a substantially sized genomic region with genotypic hallmarks of sex chromosome divergence, including in regions known to be tightly linked to the sex-determining region.Wealso foundthat expression andmolecular evolutionof genes linked to the sex-determining region did not differ substantially from genes in other parts of the genome. This suggests that the pseudoautosomal region, where recombination occurs, comprises a large portion of the sex chromosomes of S. tropicalis. These resultsmay in part explainwhy African clawed frogs have such a high incidence of polyploidization, shed light onwhy amphibians have a high rate of sex chromosome turnover, and raise questions about why homomorphic sex chromosomes are so prevalent in amphibians.