Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

Immunostimulatory activity of murine keratinocyte-derived exosomes.


Urlaub,  H.
Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society;


Wenzel,  D.
Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society;

External Resource
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)

(Supplementary material), 883KB


Kotzerke, K., Mempel, M., Aung, T., Wulf, G. G., Urlaub, H., Wenzel, D., et al. (2013). Immunostimulatory activity of murine keratinocyte-derived exosomes. Experimental Dermatology, 22(10), 650-655. doi:10.1111/exd.12230.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0014-9AAB-5
It has long been known that keratinocytes influence cutaneous immunity through secretion of soluble factors. Exosomes, small membrane vesicles of endocytotic origin, have been implicated in intercellular communication processes such as the transfer of tumor cell antigens and the activation of recipient dendritic cells (DC). However, little is known about immunomodulatory functions of keratinocyte-derived exosomes. To address this question, we analysed exosome secretion of the murine keratinocyte cell line MPEK under steady state as well as inflammatory conditions (+/- IFN). These exosomes were readily taken up by bone marrow-derived DC (BMDC) in vitro resulting in a matured phenotype, as evidenced by increased CD40 expression as well as by the production of large amounts of IL-6, IL-10 and IL-12. When the transfer of antigen-specific information through exosomes was investigated, it was found that keratinocytes took up antigen (ovalbumin) and transferred it to their exosomes. However, these antigen-harbouring exosomes failed to induce antigen-specific T cell responses via BMDC. Together, this novel biological function suggests that keratinocytes are able to direct unspecific immune processes but do not elicit specific immune responses.