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Ultra-high throughput detection of single cell beta-galactosidase activity in droplets using micro-optical lens array.

MPG-Autoren
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Lim,  J.
Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Karamitros,  C. S.
Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Konrad,  M.
Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Zitation

Lim, J., Vrignon, J., Gruner, P., Karamitros, C. S., Konrad, M., & Baret, J. C. (2013). Ultra-high throughput detection of single cell beta-galactosidase activity in droplets using micro-optical lens array. Applied Physics Letters, 103(20): 203704. doi:10.1063/1.4830046.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0014-CEA9-C
Zusammenfassung
We demonstrate the use of a hybrid microfluidic-micro-optical system for the screening of enzymatic activity at the single cell level. Escherichia coli beta-galactosidase activity is revealed by a fluorogenic assay in 100 pl droplets. Individual droplets containing cells are screened by measuring their fluorescence signal using a high-speed camera. The measurement is parallelized over 100 channels equipped with microlenses and analyzed by image processing. A reinjection rate of 1 ml of emulsion per minute was reached corresponding to more than 10(5) droplets per second, an analytical throughput larger than those obtained using flow cytometry.