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The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum

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Knispel,  Roland Wilhelm
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Nagy,  Istvan
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Baka, E., Varga, S., Hobel, C., Knispel, R. W., Fekete, C., Ivanics, M., et al. (2013). The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum. JOURNAL OF MICROBIOLOGICAL METHODS, 95(2), 145-148. doi:10.1016/j.mimet.2013.08.006.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0014-D109-8
Abstract
A transformation method yielding up to 10(4) transformants per mu g circular DNA was developed for Thermoplasma acidophilum. The method is based on a natural DNA uptake process in which T. acidophilum cells keep their integrity and turn competent at pH 3.5 and 58 degrees C. Shuttle vector maintenance could not be detected, since the used Nov(R) gyraseB gene integrated into its chromosomal counterpart by homologous recombination. (C) 2013 Elsevier B.V. All rights reserved.