Structure and RNA-binding properties of the Not1-Not2Not5 module of the yeast 
Ccr4-Not complex.

Bhaskar, V.; Roudko, V.; Basquin, J.; Sharma, K.; Urlaub, H.; Seraphin, B.; Conti, E.

doi:10.1038/nsmb.2686

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Structure and RNA-binding properties of the Not1-Not2Not5 module of the yeast Ccr4-Not complex.

MPS-Authors

/persons/resource/persons119006

Bhaskar, V.
Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons77713

Basquin, J.
Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons118921

Sharma, K.
Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15947

Urlaub, H.
Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons77867

Conti, E.
Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Bhaskar, V., Roudko, V., Basquin, J., Sharma, K., Urlaub, H., Seraphin, B., et al. (2013). Structure and RNA-binding properties of the Not1-Not2Not5 module of the yeast Ccr4-Not complex. Nature Structrural and Molecular Biology, 12(20), 1281-1288. doi:10.1038/nsmb.2686.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0014-CFD5-F

Abstract

The Ccr4-Not complex is involved in several aspects of gene expression, including mRNA decay, translational repression and transcription. We determined the 2.8-angstrom-resolution crystal structure of a 120-kDa core complex of the Saccharomyces cerevisiae Not module comprising the C-terminal arm of Not1, Not2 and Not5. Not1 is a HEAT-repeat scaffold. Not2 and Not5 have extended regions that wrap around Not1 and around their globular domains, the Not boxes. The Not boxes resemble Sm folds and interact with each other with a noncanonical dimerization surface. Disruption of the interactions within the ternary complex has severe effects on growth in vivo. The ternary complex forms a composite surface that binds poly( U) RNA in vitro, with a site at the Not5 Not box. The results suggest that the Not module forms a versatile platform for macromolecular interactions.