Nanoscopy of filamentous actin in cortical dendrites of a living mouse.

Willig, K. I.; Steffens, H.; Gregor, C.; Herholt, A.; Rossner, M. J.; Hell, S. W.

doi:10.1016/j.bpj.2013.11.1119

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Nanoscopy of filamentous actin in cortical dendrites of a living mouse.

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Willig, K. I.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Steffens, H.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Hell, S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Citation

Willig, K. I., Steffens, H., Gregor, C., Herholt, A., Rossner, M. J., & Hell, S. W. (2014). Nanoscopy of filamentous actin in cortical dendrites of a living mouse. Biophysical Journal, 106(1), L01-L03. doi:10.1016/j.bpj.2013.11.1119.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0014-F4F3-A

Abstract

We demonstrate superresolution fluorescence microscopy (nanoscopy) of protein distributions in a mammalian brain in vivo. Stimulated emission depletion microscopy reveals the morphology of the filamentous actin in dendritic spines down to 40 μm in the molecular layer of the visual cortex of an anesthetized mouse. Consecutive recordings at 43–70 nm resolution reveal dynamical changes in spine morphology.