Ran-binding protein 5 (RanBP5) is related to the nuclear transport factor 
importin-beta but interacts differently with RanBP1.

Deane, R.; Schäfer, W.; Zimmermann, H. P.; Mueller, L.; Görlich, D.; Prehn, S.; Ponstingl, H.; Bischoff, F. R.

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Ran-binding protein 5 (RanBP5) is related to the nuclear transport factor importin-beta but interacts differently with RanBP1.

MPS-Authors

/persons/resource/persons15132

Görlich, D.
Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society;

External Resource

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC232359/pdf/175087.pdf
(Publisher version)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

1922653.pdf
(Publisher version), 2MB

Supplementary Material (public)

There is no public supplementary material available

Citation

Deane, R., Schäfer, W., Zimmermann, H. P., Mueller, L., Görlich, D., Prehn, S., et al. (1997). Ran-binding protein 5 (RanBP5) is related to the nuclear transport factor importin-beta but interacts differently with RanBP1. Molecular and Cellular Biology, 17(9), 5087-5096.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0015-3C04-6

Abstract

We report the identification and characterization of a novel 124-kDa Ran binding protein, RanBP5. This protein is related to importin-beta, the key mediator of nuclear localization signal (NLS)-dependent nuclear transport. RanBP5 was identified by two independent methods: it was isolated from HeLa cells by using its interaction with RanGTP in an overlay assay to monitor enrichment, and it was also found by the yeast two-hybrid selection method with RanBP1 as bait. RanBP5 binds to RanBP1 as part of a trimeric RanBP1-Ran-RanBP5 complex. Like importin-beta, RanBP5 strongly binds the GTP-bound form of Ran, stabilizing it against both intrinsic and RanGAP1-induced GTP hydrolysis and also against nucleotide exchange. The GAP resistance of the RanBP5-RanGTP complex can be relieved by RanBP1, which might reflect an in vivo role for RanBP1. RanBP5 is a predominantly cytoplasmic protein that can bind to nuclear pore complexes. We propose that RanBP5 is a mediator of a nucleocytoplasmic transport pathway that is distinct from the importin-alpha-dependent import of proteins with a classical NLS.