Caspases mediate nucleoporin cleavage, but not early redistribution of nuclear 
transport factors and modulation of nuclear permeability in apoptosis.

Ferrando-May, E.; Cordes, V.; Biller-Ckovric, I.; Mirkovic, J.; Görlich, D.; Nicotera, P.

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Caspases mediate nucleoporin cleavage, but not early redistribution of nuclear transport factors and modulation of nuclear permeability in apoptosis.

MPS-Authors

/persons/resource/persons15132

Görlich, D.
Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society;

External Resource

http://www.nature.com/cdd/journal/v8/n5/pdf/4400837a.pdf
(Publisher version)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

1932939.pdf
(Publisher version), 361KB

Supplementary Material (public)

There is no public supplementary material available

Citation

Ferrando-May, E., Cordes, V., Biller-Ckovric, I., Mirkovic, J., Görlich, D., & Nicotera, P. (2001). Caspases mediate nucleoporin cleavage, but not early redistribution of nuclear transport factors and modulation of nuclear permeability in apoptosis. Cell Death and Differentiation, 8(5), 495-505.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0015-3D6C-7

Abstract

In eukaryotic cells, both soluble transport factors and components of the nuclear pore complex mediate protein and RNA trafficking between the nucleus and the cytoplasm. Here, we investigated whether caspases, the major execution system in apoptosis, target the nuclear pore or components of the nuclear transport machinery. Four nucleoporins, Nup153, RanBP2, Nup214 and Tpr are cleaved by caspases during apoptosis. In contrast, the nuclear transport factors, Ran, importin alpha and importin beta are not proteolytically processed, but redistribute across the nuclear envelope independently and prior to caspase activation. Also, mRNA accumulates into the nucleus before caspases become active. Microinjection experiments further revealed that early in apoptosis, the nucleus becomes permeable to dextran molecules of 70 kD molecular weight. Redistribution of import factors and mRNA, as well as nuclear permeabilisation, occur prior to caspase-mediated nucleoporin cleavage. Our findings suggest that the apoptotic programme includes modifications in the machinery responsible for nucleocytoplasmic transport, which are independent from caspase-mediated degradation of nuclear proteins.