Quantitative shotgun proteomics: considerations for a high-quality workflow in 
immunology

Meissner, Felix; Mann, Matthias

doi:10.1038/ni.2781

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Quantitative shotgun proteomics: considerations for a high-quality workflow in immunology

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Meissner, Felix
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Mann, Matthias
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Meissner, F., & Mann, M. (2014). Quantitative shotgun proteomics: considerations for a high-quality workflow in immunology. NATURE IMMUNOLOGY, 15(2), 112-117. doi:10.1038/ni.2781.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0015-7B10-6

Abstract

Proteomics based on high-resolution mass spectrometry has become a powerful tool for the analysis of protein abundance, modifications and interactions. Here we describe technical aspects of proteomics workflows, instrumentation as well as computational considerations to obtain high-quality proteomics data.