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Book Chapter

Model membrane systems


Schwille,  Petra
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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Keller, H., Worch, R., & Schwille, P. (2013). Model membrane systems. In M. Williams, & T. Daviter (Eds.), Protein-Ligand Interactions. Methods in Molecular Biology, vol 1008 (pp. 417-438). Totowa, NJ.: Humana Press.

Cite as: http://hdl.handle.net/11858/00-001M-0000-0015-7BAF-6
Model membrane systems can be classified according to their shape and origin. Depending on the shape, model membranes are compatible with a wide variety of methods, ranging from biochemical and biophysical solution assays to microscopy. So far, many applications have been focused on studying the membranes themselves, rather than membrane-associated processes like protein–protein or protein–ligand interactions. However, many model membranes with embedded proteins should be amenable to these studies, too, i.e., be suitable samples for many of the techniques discussed in this book. In this chapter, we discuss how some practical limitations that often restrict the use of model membranes can be overcome. After introducing various membrane structures, an overview is given over alternative ways of their production. Finally, several established protocols, which are suitable for beginners in the field, are described in detail.