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Journal Article

A movie of the RNA polymerase nucleotide addition cycle.


Cramer,  P.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Brueckner, F., Ortiz, J., & Cramer, P. (2009). A movie of the RNA polymerase nucleotide addition cycle. Current Opinion in Structural Biology, 19(3), 294-299. doi:10.1016/j.sbi.2009.04.005.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0015-7BBC-6
During gene transcription, RNA polymerase (Pol) passes through repetitive cycles of adding a nucleotide to the growing mRNA chain. Here we obtained a movie of the nucleotide addition cycle by combining structural information on different functional states of the Pol II elongation complex (EC). The movie illustrates the two-step loading of the nucleoside triphosphate (NTP) substrate, closure of the active site for catalytic nucleotide incorporation, and the presumed two-step translocation of DNA and RNA, which is accompanied by coordinated conformational changes in the polymerase bridge helix and trigger loop. The movie facilitates teaching and a mechanistic analysis of transcription and can be downloaded from http://www.lmb.uni-muenchen.de/cramer/pr-materials.