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Journal Article

Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex.

MPS-Authors
/persons/resource/persons128572

Söding,  J.
Research Group of Computational Biology, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons127020

Cramer,  P.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

External Resource
Fulltext (public)

1935989.pdf
(Publisher version), 878KB

Supplementary Material (public)

1935989_Suppl_1.pdf
(Supplementary material), 48KB

Citation

Jasiak, A. J., Hartmann, H., Karakasili, E., Kalocsay, M., Flatley, A., Kremmer, E., et al. (2008). Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex. The Journal of Biological Chemistry, 283, 26423-26427. doi:10.1074/jbc.M803237200.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0015-7BE8-2
Abstract
Yeast RNA polymerase (Pol) II consists of a 10-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immunoprecipitation coupled to high resolution tiling microarray analysis. We show that the genome-wide occupancy profiles for Rpb7 and the core subunit Rpb3 are essentially identical. Thus, the complete Pol II associates with DNA in vivo, consistent with functional roles of Rpb4/7 throughout the transcription cycle.