Structure of an archaeal RNA polymerase.

Kusser, A. G.; Bertero, M. G.; Naji, S.; Becker, T.; Thomm, M.; Beckmann, R.; Cramer, P.

doi:10.1016/j.jmb.2007.08.066

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Structure of an archaeal RNA polymerase.

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Cramer, P.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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Citation

Kusser, A. G., Bertero, M. G., Naji, S., Becker, T., Thomm, M., Beckmann, R., et al. (2008). Structure of an archaeal RNA polymerase. Journal of Molecular Biology, 376(2), 303-307. doi:10.1016/j.jmb.2007.08.066.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0015-7D05-1

Abstract

Related multisubunit RNA polymerases (RNAPs) carry out gene transcription in all kingdoms of life. Since structural information is limited to bacterial and eukaryotic RNAPs, we determined the cryo-electron microscopic structure of the RNAP from the thermophilic archaeon Pyrococcus furiosus at 13 Å resolution. Comparison with eukaryotic RNAP II reveals a conserved architecture, no homologues for subunits Rpb8 and Rpb9, and significant deviation in the polymerase foot, jaws, pore, and protrusion. The structural organization of the archaeal RNA polymerase serves as a reference for future structure–function analysis of the transcription mechanism and allows for evolutionary comparisons.