Structure of an archaeal RNA polymerase.

Kusser, A. G.; Bertero, M. G.; Naji, S.; Becker, T.; Thomm, M.; Beckmann, R.; Cramer, P.

doi:10.1016/j.jmb.2007.08.066

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Structure of an archaeal RNA polymerase.

MPS-Authors

/persons/resource/persons127020

Cramer, P.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

External Resource

http://www.sciencedirect.com/science/article/pii/S0022283607011485/pdfft?md5=ef8cfa5f54c20ace46e3a63268a29a5d&pid=1-s2.0-S0022283607011485-main.pdf
(出版社版)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

1936120.pdf
(出版社版), 2MB

付随資料 (公開)

1936120_Suppl_1.doc
(付録資料), 53KB

引用

Kusser, A. G., Bertero, M. G., Naji, S., Becker, T., Thomm, M., Beckmann, R., & Cramer, P. (2008). Structure of an archaeal RNA polymerase. Journal of Molecular Biology, 376(2), 303-307. doi:10.1016/j.jmb.2007.08.066.

引用: https://hdl.handle.net/11858/00-001M-0000-0015-7D05-1

要旨

Related multisubunit RNA polymerases (RNAPs) carry out gene transcription in all kingdoms of life. Since structural information is limited to bacterial and eukaryotic RNAPs, we determined the cryo-electron microscopic structure of the RNAP from the thermophilic archaeon Pyrococcus furiosus at 13 Å resolution. Comparison with eukaryotic RNAP II reveals a conserved architecture, no homologues for subunits Rpb8 and Rpb9, and significant deviation in the polymerase foot, jaws, pore, and protrusion. The structural organization of the archaeal RNA polymerase serves as a reference for future structure–function analysis of the transcription mechanism and allows for evolutionary comparisons.