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Book Chapter

Generation and Analysis of Biosensors to Measure Mechanical Forces Within Cells

MPS-Authors
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Austen,  Katharina
Grashoff, Carsten / Molecular Mechanotransduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Kluger,  Carleen
Grashoff, Carsten / Molecular Mechanotransduction, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons127894

Freikamp,  Andrea
Grashoff, Carsten / Molecular Mechanotransduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Chrostek-Grashoff,  Anna
Grashoff, Carsten / Molecular Mechanotransduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Grashoff,  Carsten
Grashoff, Carsten / Molecular Mechanotransduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Austen, K., Kluger, C., Freikamp, A., Chrostek-Grashoff, A., & Grashoff, C. (2013). Generation and Analysis of Biosensors to Measure Mechanical Forces Within Cells. In T. A. Baudino (Ed.), Methods in Molecular Biology, 1066 (pp. 169-184). New York: Springer.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0015-8057-3
Abstract
The inability to measure mechanical forces within cells has been limiting our understanding of how mechanical information is processed on the molecular level. In this chapter, we describe a method that allows the analysis of force propagation across distinct proteins within living cells using Förster resonance energy transfer (FRET)-based biosensors.