A tandem SH2 domain in transcription elongation factor Spt6 binds the 
phosphorylated RNA polymerase II C-terminal repeat domain (CTD)

Sun, M.; Larivière, L.; Dengl, S.; Mayer, A.; Cramer, P.

doi:10.1074/jbc.M110.144568

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

A tandem SH2 domain in transcription elongation factor Spt6 binds the phosphorylated RNA polymerase II C-terminal repeat domain (CTD)

MPS-Authors

/persons/resource/persons127020

Cramer, P.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

External Resource

http://www.jbc.org/content/285/53/41597.full.pdf
(Publisher version)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

1937913.pdf
(Publisher version), 3MB

Supplementary Material (public)

There is no public supplementary material available

Citation

Sun, M., Larivière, L., Dengl, S., Mayer, A., & Cramer, P. (2010). A tandem SH2 domain in transcription elongation factor Spt6 binds the phosphorylated RNA polymerase II C-terminal repeat domain (CTD). Journal of Biological Chemistry, 285(53), 41597-41603. doi:10.1074/jbc.M110.144568.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0015-81FA-D

Abstract

Spt6 is an essential transcription elongation factor and histone chaperone that binds the C-terminal repeat domain (CTD) of RNA polymerase II. We show here that Spt6 contains a tandem SH2 domain with a novel structure and CTD-binding mode. The tandem SH2 domain binds to a serine 2-phosphorylated CTD peptide in vitro, whereas its N-terminal SH2 subdomain, which we previously characterized, does not. CTD binding requires a positively charged crevice in the C-terminal SH2 subdomain, which lacks the canonical phospho-binding pocket of SH2 domains and had previously escaped detection. The tandem SH2 domain is apparently required for transcription elongation in vivo as its deletion in cells is lethal in the presence of 6-azauracil.