English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Fluorescence detection of nucleic acids and proteins in multi-component crystals.

MPS-Authors
/persons/resource/persons127020

Cramer,  P.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Kettenberger, H., & Cramer, P. (2006). Fluorescence detection of nucleic acids and proteins in multi-component crystals. Acta Crystallographica Section D: Biological Crystallography, 62(2), 146-150. doi:10.1107/S0907444905035365.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0015-834A-B
Abstract
A highly sensitive and specific assay for the rapid detection of nucleic acids in crystals of macromolecular complexes is reported. The assay is based on the observation of crystals under a fluorescence microscope after incubation with a fluorescent dye that specifically binds nucleic acids. The assay allows clear distinction between crystals of free RNA polymerase II and crystals of the polymerase bound to DNA and RNA or RNA alone, even when the nucleic acids account for only 3% of the crystalline material. Fluorescence detection was also used to monitor the diffusion and accumulation of a fluorescence-labelled polymerase-binding protein in preformed polymerase crystals. Additional experiments suggest the general applicability of these fluorescence assays for the characterization of crystal contents.