Selenomethionine incorporation in Saccharomyces cerevisiae RNA polymerase II

Bushnell, D. A.; Cramer, P.; Kornberg, R. D.

doi:10.1016/S0969-2126(00)00554-2

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Journal Article

Selenomethionine incorporation in Saccharomyces cerevisiae RNA polymerase II

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Cramer, P.
Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society;

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1943331.pdf
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Citation

Bushnell, D. A., Cramer, P., & Kornberg, R. D. (2001). Selenomethionine incorporation in Saccharomyces cerevisiae RNA polymerase II. Structure, 9(1), R11-R14. doi:10.1016/S0969-2126(00)00554-2.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0015-8719-4

Abstract

A protocol for the incorporation of SeMet into yeast proteins is described. Incorporation at a level of about 50% suffices for the location of Se sites in an anomalous difference Fourier map of the 0.5 MDa yeast RNA polymerase II. This shows the utility of the approach as an aid in the model-building of large protein complexes.