Localization of eukaryote-specific ribosomal proteins in a 5.5-angstrom cryo-EM 
map of the 80S eukaryotic ribosome.

Armache, J. P.; Jarasch, A.; Anger, A. M.; Villa, E.; Becker, T.; Bhushan, S.; Jossinet, F.; Habeck, M.; Dindar, G.; Franckenberg, S.; Marquez, V.; Mielke, T.; Thomm, M.; Berninghausen, O.; Beatrix, B.; Söding, J.; Westhof, E.; Wilson, D. N.; Beckmann, R.

doi:10.1073/pnas.1010005107

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Localization of eukaryote-specific ribosomal proteins in a 5.5-angstrom cryo-EM map of the 80S eukaryotic ribosome.

MPS-Authors

/persons/resource/persons128572

Söding, J.
Research Group of Computational Biology, MPI for Biophysical Chemistry, Max Planck Society;

External Resource

http://www.pnas.org/content/107/46/19754.full
(Publisher version)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

1944225.pdf
(Publisher version), 3MB

Supplementary Material (public)

1944225_Suppl.pdf
(Supplementary material), 3MB

Citation

Armache, J. P., Jarasch, A., Anger, A. M., Villa, E., Becker, T., Bhushan, S., et al. (2010). Localization of eukaryote-specific ribosomal proteins in a 5.5-angstrom cryo-EM map of the 80S eukaryotic ribosome. Proceedings of the National Academy of Sciences of the United States of America, 107(46), 19754-19759. doi:10.1073/pnas.1010005107.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0017-9440-E

Abstract

Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution, together with a 6.1-Å map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribosome provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.