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Energy-Resolved Ion Mobility-Mass Spectrometry - A Concept to Improve the Separation of Isomeric Carbohydrates

MPG-Autoren
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Hoffmann,  Waldemar
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Hofmann,  Johanna
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Pagel,  Kevin
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Zitation

Hoffmann, W., Hofmann, J., & Pagel, K. (2014). Energy-Resolved Ion Mobility-Mass Spectrometry - A Concept to Improve the Separation of Isomeric Carbohydrates. Journal of the American Society for Mass Spectrometry, 25(3), 471-479. doi:10.1007/s13361-013-0780-0.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0017-ABD6-1
Zusammenfassung
Recent works using ion mobility-mass spectrometry (IM-MS) have highlighted the power of this instrumental configuration to tackle one of the greatest challenges in glycomics and glycoproteomics: the existence of isobaric isomers. For a successful separation of species with identical mass but different structure via IM-MS, it is crucial to have sufficient IM resolution. In commercially available IM-MS instruments, however, this resolution is limited by the design of the instrument and usually cannot be increased at-will without extensive modifications. Here, we present a systematic approach to improve the resolving capability of IM-MS instruments using so-called energy-resolved ion mobility-mass spectrometry. The technique utilizes the fact that individual components in an isobaric mixture fragment at considerably different energies when activated in the gas phase via collision-induced dissociation (CID). As a result, certain components can be suppressed selectively at increased CID activation energy. Using a mixture of four isobaric carbohydrates, we show that each of the individual sugars can be resolved and unambiguously identified even when their drift times differ by as little as 3 %. However, the presented results also indicate that a certain difference in the gas-phase stability of the individual components is crucial for a successful separation via energy-resolved IM-MS.