The non-structural 3 (NS3) protein of dengue virus type 2 interacts with human 
nuclear receptor binding protein and is associated with alterations in membrane 
structure.

Chua, J. J. E.; Ng, M. M. L.; Chow , V. T. K.

doi:10.1016/j.virusres.2004.01.025

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The non-structural 3 (NS3) protein of dengue virus type 2 interacts with human nuclear receptor binding protein and is associated with alterations in membrane structure.

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Chua, J. J. E.
Research Group of Protein Trafficking in Synaptic Development and Function, MPI for Biophysical Chemistry, Max Planck Society;

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http://www.sciencedirect.com/science/article/pii/S0168170204000437/pdfft?md5=95eae3c5d9a60fe71e3393098d0c950d&pid=1-s2.0-S0168170204000437-main.pdf
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Citation

Chua, J. J. E., Ng, M. M. L., & Chow, V. T. K. (2004). The non-structural 3 (NS3) protein of dengue virus type 2 interacts with human nuclear receptor binding protein and is associated with alterations in membrane structure. Virus Research, 102(2), 151-163. doi:10.1016/j.virusres.2004.01.025.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0018-0AE4-1

Abstract

Flaviviral infections produce a distinct array of virus-induced intracellular membrane alterations that are associated with the flaviviral replication machinery. Currently, it is still unknown which flaviviral protein(s) is/are responsible for this induction. Using yeast two-hybrid and co-immunoprecipitation analyses, we demonstrated that the NS3 protein of dengue virus type 2 interacted specifically with nuclear receptor binding protein (NRBP), a host cellular protein that influences trafficking between the endoplasmic reticulum (ER) and Golgi, and that interacts with Rac3, a member of the Rho-GTPase family. Co-expression of NS3 and NRBP in baby hamster kidney cells exhibited significant subcellular co-localization, and revealed the redistribution of NRBP from the cytoplasm to the perinuclear region. Furthermore, a set of membrane structures affiliated with the rough ER at the perinuclear region was induced in cells transfected with NS3. These structures are reminiscent of the virus-induced convoluted membranes previously observed in flavivirus-infected cells. This interaction between dengue viral and host cell proteins as well as the formation of the NS3-induced membrane structures suggest that NS3 may subvert the role of NRBP in ER-Golgi trafficking.