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Electroporation of nucleic acids into prokaryotic and eukaryotic cells by square wave pulses

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Koenen,  Michael
Molecular anatomy of the neuromuscular junction, Max Planck Institute for Medical Research, Max Planck Society;
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Working Group Witzemann / Koenen, Max Planck Institute for Medical Research, Max Planck Society;
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Hänze, J., Fischer, L., Koenen, M., Worgall, S., & Rascher, W. (1998). Electroporation of nucleic acids into prokaryotic and eukaryotic cells by square wave pulses. Biotechnology Techniques, 12(2), 159-163. doi:10.1023/A:1008800903452.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0019-A4CF-B
Abstract
A novel electroporator is presented that generates adjustable square wave pulses with a maximal pulse strength of 2500 V / 100 A and a pulse length between 0.1 and 15 ms and that efficiently transfects prokaryotic and eukaryotic cells. Various factors influencing DNA transfer into tissue culture cells, embryonic stem cells and E. coli K12 cells were optimized and the respective electrical pulse profiles monitored.