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3-Deoxy-octulosonic Acid-Containing Trisaccharide Fragment of an Unusual Core Type of Some Hafnia alvei Lipopolysaccharides

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Dabrowski,  Ursula
Department of Organic Chemistry, Max Planck Institute for Medical Research, Max Planck Society;

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Dabrowski,  Janusz
Department of Organic Chemistry, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Katzenellenbogen, E., Gamian, A., Romanowska, E., Dabrowski, U., & Dabrowski, J. (1993). 3-Deoxy-octulosonic Acid-Containing Trisaccharide Fragment of an Unusual Core Type of Some Hafnia alvei Lipopolysaccharides. Biochemical and Biophysical Research Communications, 194(3), 1058-1064. doi:10.1006/bbrc.1993.1929 Get.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0019-AA1A-6
Abstract
The 3-deoxy-octulosonic acid-containing trisaccharide was isolated from the carbohydrate material obtained after the acid hydrolysis of lipopolysaccharides of Hafnia alvei strains 2 and 1211 using gel filtration. For purification the trisaccharide fraction was rechromatographed on a Bio-Gel P2 column. On the basis of sugar and methylation analyses and 1H NMR spectroscopy the trisaccharide was identified as 3-deoxy-7-O-(6-O-acetyl-alpha-D-galactopyranosyl)-4-O-(alpha-L-glycero-D - mannoheptopyranosyl)-D-manno-2-octulopyranosic acid. The occurrence of such bifurcate Kdo-containing structure has not been previously reported in Enterobacteriaceae.