Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Heterologous expression of EPS-genes in Erwinia amylovora and Erwinia stewartii

MPG-Autoren
/persons/resource/persons92154

Bellemann,  Peter
Research Group Prof. Dr. Geider, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons93045

Geider,  Klaus
Research Group Prof. Dr. Geider, Max Planck Institute for Medical Research, Max Planck Society;

Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Bernhard, F., Bellemann, P., Bugert, P., Coplin, D. L., & Geider, K. (1993). Heterologous expression of EPS-genes in Erwinia amylovora and Erwinia stewartii. Acta Horticulturae, 338, 265-272. doi:10.17660/ActaHortic.1993.338.41.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0019-AACE-3
Zusammenfassung
A gene cluster for exopolysaccharide (EPS) synthesis of Erwinia amylovora was cloned in cosmid pEA109 and identified by complementation of Erwinia stewartii mutants deficient in EPS−production (cps). A corresponding cluster from E. stewartii (pES2144) complemented EPS−mutants of E. amylovora (ams). Two EPS−dependent phages were used to characterize EPS synthesized in heterologously complemented mutants. An E. stewartii cpsC mutant carrying pEA109 produced mainly amylovoran in contrast to a cpsE−mutant complemented with pEA109 or to E. amylovora mutant D4 carrying pES2144, which produced predominantly stewartan, the EPS of E. stewartii. Lectin binding could also be used to detect amylovoran synthesis by heterologously complemented Erwinia mutants. Heterologous complementation of EPS−synthesis restored virulence to E. stewartii mutants but not to E. amylovora mutants. In addition to functional complementation, comparison of parts of nucleotide sequences from the ams and cps regions confirmed homologies between ams and cps genes