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Derivatization of ribosomes and of tRNA with an undecagold cluster: crystallographic and functional studies

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Jahn,  Werner
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Tichelaar,  Willem
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Weinstein, S., Jahn, W., Laschever, M., Arad, T., Tichelaar, W., Haider, M., et al. (1992). Derivatization of ribosomes and of tRNA with an undecagold cluster: crystallographic and functional studies. Journal of Crystal Growth, 122(1), 286-292. doi:10.1016/0022-0248(92)90257-J.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0019-AB45-B
Abstract
An undecagold cluster was covalently attached to whole ribosomes and to their small and large subunits prior to their crystallization. X-ray crystallographic data were collected from crystals of the first two. The same cluster was bound to tRNAphe fromE. coli at base 47. It was found that the modified tRNA molecule binds to the ribosome and can be aminoacylated by its cognate synthetase. The gold cluster modified tRNAphe may be used for phasing diffraction data of crystals of complexes containing it, mimicking defined states in the process of protein biosynthesis.