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Journal Article

Isolation and characterization of the complete human β-myosin heavy chain gene


Vosberg,  Hans−Peter
Max Planck Society;

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Diederich, K. W.., Eisele, I., Ried, T., Jaenicke, T., Lichter, P., & Vosberg, H. (1989). Isolation and characterization of the complete human β-myosin heavy chain gene. Human Genetics, 81(3), 214-220. doi:10.1007/BF00278991.

Cite as: http://hdl.handle.net/11858/00-001M-0000-0019-ADEF-0
The entire gene coding for the human beta-myosin heavy chain has been isolated from genomic EMBL3A phage libraries by chromosomal walking starting from clone gMHC-1, reported earlier (Appelhans and Vosberg 1983). gMHC-1 has been shown to carry coding information for the C-terminal two-thirds of beta-myosin heavy chain, which is expressed in cardiac muscle and in slow skeletal muscle fibers (Lichter et al. 1986). Three DNA clones were identified as overlapping with gMHC-1 by restriction mapping and DNA sequencing. They span a 30-kb region in the genome. About 22 kb extend from the initiation codon ATG to the poly(A) addition site. The clones include about 4 kb of 5' flanking sequences upstream of the promoter. Comparisons of beta- and alpha-myosin heavy chain sequences indicate that gene duplication of the cardiac myosin heavy chain isogenes preceded the mammalian species differentiation.