Imaging the activity of Ras superfamily GTPase proteins in small subcellular 
compartments in neurons

Oliveira, A. F.; Yasuda, R.

doi:10.1007/978-1-62703-622-1_9

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Imaging the activity of Ras superfamily GTPase proteins in small subcellular compartments in neurons

MPS-Authors

Yasuda, R.
Max Planck Florida Institute for Neuroscience, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Oliveira, A. F., & Yasuda, R. (2014). Imaging the activity of Ras superfamily GTPase proteins in small subcellular compartments in neurons. Methods in Molecular Biology, 1071(2014), 109-128. doi:10.1007/978-1-62703-622-1_9.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0019-00AF-F

Abstract

Resolving the spatiotemporal dynamics of intracellular signaling is important for understanding the molecular mechanisms of various cellular processes induced by extracellular signals. Two-photon fluorescence lifetime imaging microscopy (2pFLIM) in combination with a fluorescence resonance energy transfer (FRET)-based signaling sensors allows one to image signaling within small subcellular compartments, such as dendritic spines of neurons, with high sensitivity and spatiotemporal resolution. In this protocol, we describe the procedures and equipment required for imaging intracellular signaling activity, with a particular focus on signaling mediated by the Ras superfamily of small GTPase proteins.