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Journal Article

Super-resolution microscopy of mitochondria.


Jakobs,  S.
Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society;


Wurm,  C. A.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Jakobs, S., & Wurm, C. A. (2014). Super-resolution microscopy of mitochondria. Current Opinion in Chemical Biology, 20, 9-15. doi:10.1016/j.cbpa.2014.03.019.

Cite as: http://hdl.handle.net/11858/00-001M-0000-0019-8B7B-F
Mitochondria, the powerhouses of the cell, are essential organelles in eukaryotic cells. With their complex inner architecture featuring a smooth outer and a highly convoluted inner membrane, they are challenging objects for microscopy. The diameter of mitochondria is generally close to the resolution limit of conventional light microscopy, rendering diffraction-unlimited super-resolution light microscopy (nanoscopy) for imaging submitochondrial protein distributions often mandatory. In this review, we discuss what can be expected when imaging mitochondria with conventional diffraction-limited and diffraction-unlimited microscopy. We provide an overview on recent studies using super-resolution microscopy to investigate mitochondria and discuss further developments and challenges in mitochondrial biology that might by addressed with these technologies in the future.