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Journal Article

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.

MPS-Authors
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Mandad,  S.
Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society;

/persons/resource/persons15947

Urlaub,  H.
Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society;

External Resource

http://www.sciencemag.org/content/344/6187/1023.full.pdf
(Publisher version)

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Supplementary Material (public)

2033869_Suppl_1.pdf
(Supplementary material), 2MB

2033869_Suppl_2.pdf
(Supplementary material), 21MB

2033869_Suppl_3.xlsx
(Supplementary material), 845KB

2033869_Suppl_4.xlsx
(Supplementary material), 416KB

2033869_Suppl_5.xlsx
(Supplementary material), 762KB

2033869_Suppl_6.mov
(Supplementary material), 136MB

Citation

Wilhelm, B. G., Mandad, S., Truckenbrodt, S., Kröhnert, K., Schäfer, C., Rammner, B., et al. (2014). Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins. Science, 344(6187), 1023-1028. doi:10.1126/science.1252884.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0019-BF79-E
Abstract
Synaptic vesicle recycling has long served as a model for the general mechanisms of cellular trafficking. We used an integrative approach, combining quantitative immunoblotting and mass spectrometry to determine protein numbers; electron microscopy to measure organelle numbers, sizes, and positions; and super-resolution fluorescence microscopy to localize the proteins. Using these data, we generated a three-dimensional model of an "average" synapse, displaying 300,000 proteins in atomic detail. The copy numbers of proteins involved in the same step of synaptic vesicle recycling correlated closely. In contrast, copy numbers varied over more than three orders of magnitude between steps, from about 150 copies for the endosomal fusion proteins to more than 20,000 for the exocytotic ones.