English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Profilin 1 is essential for retention and metabolism of mouse hematopoietic stem cells in bone marrow

MPS-Authors
/persons/resource/persons77774

Böttcher,  Ralph T.
Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society;

External Ressource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Zheng, J., Lu, Z., Kocabas, F., Böttcher, R. T., Costell, M., Kang, X., et al. (2014). Profilin 1 is essential for retention and metabolism of mouse hematopoietic stem cells in bone marrow. BLOOD, 123(7), 992-1001. doi:10.1182/blood-2013-04-498469.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0019-D0C7-3
Abstract
How stem cells interact with the microenvironment to regulate their cell fates and metabolismis largely unknown. Here we demonstrated that the deletion of the cytoskeleton modulating protein profilin 1 (pfn1) in hematopoietic stem cell (HSCs) led to bone marrow failure, loss of quiescence, and mobilization and apoptosis of HSCs in vivo. A switch from glycolysis to mitochondrial respiration with increased reactive oxygen species (ROS) level was also observed in HSCs on pfn1 deletion. Importantly, treatment of pfn1-deficient mice with the antioxidant N-acetyl-L-cysteine reversed the ROS level and loss of quiescence of HSCs, suggesting that the metabolism is mechanistically linked to the cell cycle quiescence of stem cells. The actin-binding and proline-binding activities of pfn1 are required for its function in HSCs. Our study provided evidence that pfn1 at least partially acts through the axis of pfn1/G alpha 13/EGR1 to regulate stem cell retention and metabolism in the bone marrow.