A STED microscope designed for routine biomedical applications.

Görlitz, F.; Hoyer, P.; Falk , H.; Kastrup, L.; Engelhardt, J.; Hell, S. W.

doi:10.2528/PIER14042708

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Journal Article

A STED microscope designed for routine biomedical applications.

MPS-Authors

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Hoyer, P.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Kastrup, L.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Engelhardt, J.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Hell, S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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http://www.jpier.org/PIER/pier147/04.14042708.pdf
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2035117.pdf
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Citation

Görlitz, F., Hoyer, P., Falk, H., Kastrup, L., Engelhardt, J., & Hell, S. W. (2014). A STED microscope designed for routine biomedical applications. Progress in Electromagnetics Research, 147, 57-68. doi:10.2528/PIER14042708.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0019-D43F-C

Abstract

We present a multi-color STED fluorescence microscope providing far-field optical resolution down to 20 nm for biomedical research. The optical design comprises fiber lasers, beam scanners, and a set of active and passive polarizing elements that cooperatively yield an optically robust system for routinely imaging samples at subdiffraction length scales.