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Journal Article

Microfluidic cryofixation for correlative microscopy.

MPS-Authors
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Mejia,  Y. X.
Research Group of Biological Micro- and Nanotechnology, MPI for biophysical chemistry, Max Planck Society;

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Burg,  T. P.
Research Group of Biological Micro- and Nanotechnology, MPI for biophysical chemistry, Max Planck Society;

External Ressource
Fulltext (public)

2042461.pdf
(Publisher version), 2MB

Supplementary Material (public)

2042461_Suppl.pdf
(Supplementary material), 2MB

Citation

Mejia, Y. X., Feindt, H., Zhang, D., Steltenkamp, S., & Burg, T. P. (2014). Microfluidic cryofixation for correlative microscopy. Lab on a Chip, 14(17), 3281-3284. doi:10.1039/c4lc00333k.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0019-ED3F-1
Abstract
Cryofixation yields outstanding ultrastructural preservation of cells for electron microscopy, but current methods disrupt live cell imaging. Here we demonstrate a microfluidic approach that enables cryofixation to be performed directly in the light microscope with millisecond time resolution and at atmospheric pressure. This will provide a link between imaging/stimulation of live cells and post-fixation optical, electron, or X-ray microscopy.