Copy number variants and selective sweeps in natural populations of the house 
mouse (Mus musculus domesticus)

Bryk, Jarosłav; Tautz, Diethard

doi:10.3389/fgene.2014.00153

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Copy number variants and selective sweeps in natural populations of the house mouse (Mus musculus domesticus)

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Bryk, Jarosłav
Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Tautz, Diethard
Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Citation

Bryk, J., & Tautz, D. (2014). Copy number variants and selective sweeps in natural populations of the house mouse (Mus musculus domesticus). Frontiers in Genetics, 5(153): 153. doi:10.3389/fgene.2014.00153.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0023-F51B-C

Abstract

Copy–numbervariants(CNVs)mayplayanimportantroleinearlyadaptations,potentiallyfacilitatingrapiddivergenceofpopulations.WedescribeanapproachtostudythisquestionbyinvestigatingCNVspresentinnaturalpopulationsofmiceintheearlystagesofdivergenceandtheirinvolvementinselectivesweeps.Wehaveanalyzedindividualsfromtworecentlydivergednaturalpopulationsofthehousemouse(Musmusculusdomesticus)fromGermanyandFranceusingcustom,high–density,comparativegenomehybridizationarrays(CGH)thatcoveredalmost164Mband2444genes.Onethousandeighthundredandsixtyoneofthosegeneswepreviouslyidentifiedasdifferentiallyexpressedbetweenthesepopulations,whiletheexpressionoftheremaininggeneswasinvariant.Intotal,weidentified1868CNVsacrossall10samples,200bpto600kbinsizeandaffecting424genicregions.RoughlytwothirdsofallCNVsfoundweredeletions.WefoundnoenrichmentofCNVsamongthedifferentiallyexpressedgenesbetweenthepopulationscomparedtotheinvariantones,noranymeaningfulcorrelationbetweenCNVsandgeneexpressionchanges.AmongtheCNVgenes,wefoundcellularcomponentgeneontologycategoriesofthesynapseoverrepresentedamongallthe2444genestested.ToinvestigatepotentialadaptivesignificanceoftheCNVregions,weselectedsixthatshowedlargedifferencesinfrequencyofCNVsbetweenthetwopopulationsandanalyzedvariationinatleasttwomicrosatellitessurroundingthelociinasampleof46unrelatedanimalsfromthesamepopulationscollectedinfieldtrappings.Weidentifiedtwolociwithlargedifferencesinmicrosatelliteheterozygosity(Sfi1andGlo1/Dnahc8regions)andonelocuswithlowvariationacrossthepopulations(Cmah),thussuggestingthatthesegenomicregionsmighthaverecentlyundergoneselectivesweeps.Interestingly,theGlo1CNVhaspreviouslybeenimplicatedinanxiety–likebehaviorinmice,suggestingadifferentialevolutionofabehavioraltrait.