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Journal Article

Staining and embedding the whole mouse brain for electron microscopy

MPS-Authors
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Mikula,  Shawn
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Binding,  Jonas
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Denk,  Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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http://dx.doi.org/10.1038/nmeth.2213
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Citation

Mikula, S., Binding, J., & Denk, W. (2012). Staining and embedding the whole mouse brain for electron microscopy. Nature methods, 9(12), 1198-1201. doi:10.1038/nmeth.2213.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-0847-D
Abstract
The development of methods for imaging large contiguous volumes with the electron microscope could allow the complete mapping of a whole mouse brain at the single−axon level. We developed a method based on prolonged immersion that enables staining and embedding of the entire mouse brain with uniform myelin staining and a moderate preservation of the tissue's ultrastructure. We tested the ability to follow myelinated axons using serial block−face electron microscopy