English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy

MPS-Authors
/persons/resource/persons94543

Niedworok,  Christian
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons123350

Schwarz,  Inna
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons94038

Ledderose,  Julia
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons118183

Giese,  Günter
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons124404

Schwarz,  Martin K.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

External Ressource
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Niedworok, C., Schwarz, I., Ledderose, J., Giese, G., Conzelmann, K., & Schwarz, M. K. (2012). Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy. Cell Reports, 2(5), 1375-1386. doi:10.1016/j.celrep.2012.10.008.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0024-0852-4
Abstract
Cellular resolution three−dimensional (3D) visualization of defined, fluorescently labeled long−range neuronal networks in the uncut adult mouse brain has been elusive. Here, a virus−based strategy is described that allowed fluorescent labeling of centrifugally projecting neuronal populations in the ventral forebrain and their directly, monosynaptically connected bulbar interneurons upon a single stereotaxic injection into select neuronal populations. Implementation of improved tissue clearing combined with light−sheet fluorescence microscopy permitted imaging of the resulting connectivity maps in a single whole−brain scan. Subsequent 3D reconstructions revealed the exact distribution of the diverse neuronal ensembles monosynaptically connected with distinct bulbar interneuron populations. Moreover, rehydratation of brains after light−sheet fluorescence imaging enabled the immunohistochemical identification of synaptically connected neurons. Thus, this study describes a method for identifying monosynaptic connectivity maps from distinct, virally labeled neuronal populations that helps in better understanding of information flow in neural systems