English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo

MPS-Authors
/persons/resource/persons94395

Mittmann,  Wolfgang
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95138

Schaefer,  Andreas T.
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons128986

Denk,  Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

http://www.nature.com/neuro/journal/v14/n8/pdf/nn.2879.pdf
(Any fulltext)

http://dx.doi.org/10.1038/nn.2879
(Any fulltext)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Mittmann, W., Wallace, D. J., Czubayko, U., Herb, J. T., Schaefer, A. T., Looger, L. L., et al. (2011). Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo. Nature Neuroscience, 14(8), 1089-1093. doi:10.1038/nn.2879.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-1E21-5
Abstract
Multiphoton imaging (MPI) is widely used for recording activity simultaneously from many neurons in superficial cortical layers in vivo. We combined regenerative amplification multiphoton microscopy (RAMM) with genetically encoded calcium indicators to extend MPI of neuronal population activity into layer 5 (L5) of adult mouse somatosensory cortex. We found that this approach could be used to record and quantify spontaneous and sensory−evoked activity in populations of L5 neuronal somata located as much as 800 μm below the pia. In addition, we found that RAMM could be used to simultaneously image activity from large (80) populations of apical dendrites and follow these dendrites down to their somata of origin