Rapid construction in yeast of complex targeting vectors for gene manipulation 
in the mouse

Storck, Thorsten; Storck, U.; Kolhekar, Rohini; Sprengel, Rolf; Seeburg, Peter H.

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse

MPS-Authors

/persons/resource/persons95439

Sprengel, Rolf
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95292

Seeburg, Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

http://nar.oupjournals.org/cgi/reprint/24/22/4594
(Any fulltext)

http://dx.doi.org/10.1093/nar/24.22.4373
(Any fulltext)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Storck, T., Storck, U., Kolhekar, R., Sprengel, R., & Seeburg, P. H. (1996). Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse. Nucleic Acids Research (London), 24(22), 4594-4596. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/8948655.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-213B-A

Abstract

Targeting vectors for embryonic stem (ES) cells typically contain a mouse gene segment of >7 kb with the neo gene inserted for positive selection of the targeting event. More complex targeting vectors carry additional genetic elements (e.g. lacZ, loxP, point mutations). Here we use homologous recombination in yeast to construct targeting vectors for the incorporation of genetic elements (GEs) into mouse genes. The precise insertion of GEs into any position of a mouse gene segment cloned in an Escherichia coli/yeast shuttle vector is directed by short recombinogenic arms (RAs) flanking the GEs. In this way, complex targeting vectors can be engineered with considerable ease and speed, obviating extensive gene mapping in search for suitable restriction sites