Relative abundance of subunit mRNAs determines gating and Ca2+ permeability of 
AMPA receptors in principal neurons and interneurons in rat CNS.

Geiger, Jörg R. P.; Melcher, Thorsten; Koh, Duk Su; Sakmann, Bert; Seeburg, Peter H.; Jonas, Peter; Monyer, Hannah

doi:10.1016/0896-6273(95)90076-4

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Relative abundance of subunit mRNAs determines gating and Ca2+ permeability of AMPA receptors in principal neurons and interneurons in rat CNS.

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Melcher, Thorsten
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Koh, Duk Su
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Sakmann, Bert
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Seeburg, Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Jonas, Peter
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Monyer, Hannah
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Geiger, J. R. P., Melcher, T., Koh, D. S., Sakmann, B., Seeburg, P. H., Jonas, P., et al. (1995). Relative abundance of subunit mRNAs determines gating and Ca2+ permeability of AMPA receptors in principal neurons and interneurons in rat CNS. Neuron, 15(1), 193-204. doi:10.1016/0896-6273(95)90076-4.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-21CF-B

Abstract

Recording of glutamate-activated currents in membrane patches was combined with RT-PCR-mediated AMPA receptor (AMPAR) subunit mRNA analysis in single identified cells of rat brain slices. Analysis of AMPARs in principal neurons and interneurons of hippocampus and neocortex and in auditory relay neurons and Bergmann glial cells indicates that the GluR-B subunit in its flip version determines formation of receptors with relatively slow gating, whereas the GluR-D subunit promotes assembly of more rapidly gated receptors. The relation between Ca2+ permeability of AMPAR channels and the relative GluR-B mRNA abundance is consistent with the dominance of this subunit in determining the Ca2+ permeability of native receptors. The results suggest that differential expression of GluR-B and GluR-D subunit genes, as well as splicing and editing of their mRNAs, account for the differences in gating and Ca2+ permeability of native AMPAR channels.