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Journal Article

Pharmacology of the nicotinic acetylcholine receptor from fetal rat muscle expressed in Xenopus Oocytes

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Witzemann,  Veit
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Cooper, J. C., Gutbrod, O., Witzemann, V., & Methfessel, C. (1996). Pharmacology of the nicotinic acetylcholine receptor from fetal rat muscle expressed in Xenopus Oocytes. European Journal of Pharmacology, 309(3), 287-298. doi:10.1016/0014-2999(96)00294-4.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-428B-C
Abstract
The fetal rat muscle nicotinic acetylcholine receptor was expressed in Xenopus oocytes. Using the voltage-clamp technique, the response to a range of agonists was measured, listed in order of (decreasing) activity efficacy: anatoxin >__ epibatidine > acetylcholine > DMPP (1,1-dimethyl-4-phenylpiperazinium) >> cytisine > pyrantel > nicotine > coniine > tubocurare > lobeline. The agonist responses were compared with the steric and electrostatic properties of the molecules, using molecular modelling. Single-channel currents were measured in outside-out patches for acetylcholine, nicotine, cytisine, anatoxin and epibatidine. The conductance of the single channels was independent of the type of agonist. The mean open times were characteristic of the agonist applied. Tubocurare, better known for its antagonist properties, was also a partial agonist. Single-channel currents were also observed for tubocurare, and for methyllycaconitine in patches with a very high density of the muscle nicotinic acetylcholine receptor, and these were blocked by ~x-bungarotoxin. The agonist properties of physostigmine, galanthamine and their methyl derivatives were also investigated. The conductance of the channels observed in outside-out patches was similar to that obtained for the classical agonists. The single-channel currents observed for physostigmine, galanthamine and their methyl derivatives were blocked by a-bungarotoxin, methyllycaconitine and mecamylamine, in contrast to previously reported studies on neuronal and adult muscle nicotinic acetylcholine receptors.