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High-throughput phenotypic assessment of cardiac physiology in four commonly used inbred mouse strains


Wurst,  Wolfgang
Max Planck Institute of Psychiatry, Max Planck Society;
Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health;

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Moreth, K., Fischer, R., Fuchs, H., Gailus-Durner, V., Wurst, W., Wurst, W., et al. (2014). High-throughput phenotypic assessment of cardiac physiology in four commonly used inbred mouse strains. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY, 184(6), 763-775. doi:10.1007/s00360-014-0830-3.

Mice with genetic alterations are used in heart research as model systems of human diseases. In the last decade there was a marked increase in the recognition of genetic diversity within inbred mouse strains. Increasing numbers of inbred mouse strains and substrains and analytical variation of cardiac phenotyping methods require reproducible, high-throughput methods to standardize murine cardiovascular physiology. We describe methods for non-invasive, reliable, easy and fast to perform echocardiography and electrocardiography on awake mice. This method can be used for primary screening of the murine cardiovascular system in large-scale analysis. We provide insights into the physiological divergence of C57BL/6N, C57BL/6J, C3HeB/FeJ and 129P2/OlaHsd mouse hearts and define the expected normal values. Our report highlights that compared to the other three strains tested C57BL/6N hearts reveal features of heart failure such as hypertrophy and reduced contractile function. We found several features of the mouse ECG to be under genetic control and obtained several strain-specific differences in cardiac structure and function.