Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Simple Derivation of Transgene-Free iPS Cells by a Dual Recombinase Approach

MPG-Autoren
/persons/resource/persons80599

Wurst,  Wolfgang
Max Planck Institute of Psychiatry, Max Planck Society;
Institute of Developmental Genetics, Helmholtz Zentrum München;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Pertek, A., Meier, F., Irmler, M., Beckers, J., Skylaki, S., Endele, M., et al. (2014). Simple Derivation of Transgene-Free iPS Cells by a Dual Recombinase Approach. MOLECULAR BIOTECHNOLOGY, 56(8), 697-713. doi:10.1007/s12033-014-9748-y.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0024-682C-1
Zusammenfassung
Mammalian cells can be reprogrammed into induced pluripotent stem cells (iPSCs), a valuable tool for in vitro disease modeling and regenerative medicine. These applications demand for iPSCs devoid of reprogramming factor transgenes, but current procedures for the derivation of transgene-free iPSCs are inefficient and cumbersome. Here, we describe a new approach for the simple derivation of transgene-free iPSCs by the sequential use of two DNA recombinases, C31 Integrase and Cre, to control the genomic insertion and excision of a single, non-viral reprogramming vector. We show that such transgene-free iPSCs exhibit gene expression profiles and pluripotent developmental potential comparable to genuine, blastocyst-derived embryonic stem cells. As shown by a reporter iPSC line for the differentiation into midbrain dopaminergic neurons, the dual recombinase approach offers a simple and efficient way to derive transgene-free iPSCs for studying disease mechanisms and cell replacement therapies.