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Journal Article

Generation of targeted mouse mutants by embryo microinjection of TALENs


Wurst,  Wolfgang
Max Planck Institute of Psychiatry, Max Planck Society;
Institute of Develomental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health;

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Wefers, B., Ortiz, O., Wurst, W., & Kuehn, R. (2014). Generation of targeted mouse mutants by embryo microinjection of TALENs. METHODS, 69(1), 94-101. doi:10.1016/j.ymeth.2014.01.002.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0024-6104-6
Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes. (C) 2014 Elsevier Inc. All rights reserved.